Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | KTY107Y | Biochemistry Laboratory Techniques | Elective | 1 | 1 | 6 |
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Level of Course Unit |
Second Cycle |
Objectives of the Course |
Understanding the rules to be considered while working in the research laboratory and the features and purposes of use of the devices used in general |
Name of Lecturer(s) |
Prof. Dr. Engin ŞAHİN |
Learning Outcomes |
1 | 1. Understanding the purpose of the devices and the general features of the devices
2. Comprehending chromatographic methods. | 2 | Comprehending chromatographic methods. |
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Mode of Delivery |
Normal Education |
Prerequisites and co-requisities |
No. |
Recommended Optional Programme Components |
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Course Contents |
Cleaning in the biochemistry laboratory, Storage of chemicals and solutions, Buffer solutions, Optical methods, Spectrophotometer, Protein determination methods, IR spectroscopy, Polarimeter, Chromographical methods (Adsorption, Gel-filtration, Ion exchange, thin layer, paper, affinity, column, gas and liquid chromatography), Electrophoresis, Centrifugation |
Weekly Detailed Course Contents |
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1 | Cleaning in the lab | | | 2 | Storage of chemicals and solutions | | | 3 | buffer solutions | | | 4 | Optical methods | | | 5 | Spectrophotometer | | | 6 | Protein determination methods | | | 7 | IR spectroscopy | | | 8 | Mid-term examination | | | 9 | Polarimeter | | | 10 | General properties of chromatographic methods | | | 11 | Adsorption chromatography | | | 12 | Gel-filtration chromatography | | | 13 | Ion exchange chromatography | | | 14 | Thin sheet, paper and column chromatography | | | 15 | Gas and liquid chromatography | | |
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Recommended or Required Reading |
Biochemistry laboratory lecture notes İrfan Küfrevioğlu, |
Planned Learning Activities and Teaching Methods |
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Assessment Methods and Criteria | |
Midterm Examination | 1 | 100 | SUM | 100 | |
Final Examination | 1 | 100 | SUM | 100 | Term (or Year) Learning Activities | 40 | End Of Term (or Year) Learning Activities | 60 | SUM | 100 |
| Language of Instruction | Turkish | Work Placement(s) | |
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Workload Calculation |
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Midterm Examination | 1 | 18 | 18 |
Final Examination | 1 | 19 | 19 |
Field Work | 14 | 3 | 42 |
Criticising Paper | 15 | 3 | 45 |
Self Study | 14 | 4 | 56 |
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Contribution of Learning Outcomes to Programme Outcomes |
LO1 | 2 | 1 | 2 | 5 | 5 | 5 | 3 | 4 | 4 | 4 | 5 | 1 | 2 | 3 | 3 | 3 | 2 | LO2 | 1 | 2 | 3 | 5 | 5 | 5 | 2 | 4 | 2 | 5 | 1 | 4 | 4 | 3 | 2 | 5 | 4 |
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* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
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