| Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | | KTY129 | Biochemistry Laboratory Techniques | Elective | 1 | 1 | 6 |
|
| Level of Course Unit |
| Second Cycle |
| Objectives of the Course |
| Understanding the rules to be considered while working in the research laboratory and the features and purposes of use of the devices used in general |
| Name of Lecturer(s) |
| Prof. Dr. Engin ŞAHİN |
| Learning Outcomes |
| 1 | 1. Understanding the purpose of the devices and the general features of the devices
2. Comprehending chromatographic methods. | | 2 | Comprehending chromatographic methods. |
|
| Mode of Delivery |
| Normal Education |
| Prerequisites and co-requisities |
| No. |
| Recommended Optional Programme Components |
|
| Course Contents |
| Cleaning in the biochemistry laboratory, Storage of chemicals and solutions, Buffer solutions, Optical methods, Spectrophotometer, Protein determination methods, IR spectroscopy, Polarimeter, Chromographical methods (Adsorption, Gel-filtration, Ion exchange, thin layer, paper, affinity, column, gas and liquid chromatography), Electrophoresis, Centrifugation |
| Weekly Detailed Course Contents |
|
| 1 | Cleaning in the lab | | | | 2 | Storage of chemicals and solutions | | | | 3 | buffer solutions | | | | 4 | Optical methods | | | | 5 | Spectrophotometer | | | | 6 | Protein determination methods | | | | 7 | IR spectroscopy | | | | 8 | Mid-term examination | | | | 9 | Polarimeter | | | | 10 | General properties of chromatographic methods | | | | 11 | Adsorption chromatography | | | | 12 | Gel-filtration chromatography | | | | 13 | Ion exchange chromatography | | | | 14 | Thin sheet, paper and column chromatography | | | | 15 | Gas and liquid chromatography | | |
|
| Recommended or Required Reading |
| Biochemistry laboratory lecture notes İrfan Küfrevioğlu, |
| Planned Learning Activities and Teaching Methods |
|
| Assessment Methods and Criteria | |
| Midterm Examination | 1 | 100 | | SUM | 100 | |
| Final Examination | 1 | 100 | | SUM | 100 | | Term (or Year) Learning Activities | 40 | | End Of Term (or Year) Learning Activities | 60 | | SUM | 100 |
| | Language of Instruction | | Turkish | | Work Placement(s) | |
|
| Workload Calculation |
|
| Midterm Examination | 1 | 18 | 18 |
| Final Examination | 1 | 19 | 19 |
| Field Work | 14 | 3 | 42 |
| Criticising Paper | 15 | 3 | 45 |
| Self Study | 14 | 4 | 56 |
|
| Contribution of Learning Outcomes to Programme Outcomes |
| LO1 | 2 | 1 | 2 | 5 | 5 | 5 | 3 | 4 | 4 | 4 | 5 | 1 | 2 | 3 | 3 | 3 | 2 | | LO2 | 1 | 2 | 3 | 5 | 5 | 5 | 2 | 4 | 2 | 5 | 1 | 4 | 4 | 3 | 2 | 5 | 4 |
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| * Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
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